von Willebrand Factor Collagen Binding
The next evolution in von Willebrand Disease (VWD) diagnostics, Versiti’s novel Collagen IV Binding Assay, is now available for clinical order. In vivo, collagen binding by VWF facilitates platelet adhesion at sites of blood vessel injury. The Collagen III Binding (VWF:CB3) (order code: 1281) and Collagen IV Binding (VWF:CB4) (order code: 1280) assays are supplemental functional assays that specifically evaluates the ability of a patient’s VWF to support platelet adhesion.
Discrepancy between Collagen IV Binding and VWF antigen levels in the absence of a multimer defect could indicate type 2M VWD due to defect in the A1 domain. Similarly, discrepancy between Collagen III Binding and VWF antigen levels is a sensitive screen for deficiency of large VWF multimers, but if multimer distribution is normal, could indicate type 2M VWD due to defect in the A3 domain.
Additionally, Versiti is pleased to offer the Collagen Binding Profile (order code: 1279), designed to identify rare individuals with isolated defects in the A3 domain that binds type I and III collagen, and even rarer individuals with defects in the A1 domain that binds types IV and VI collagen.