Factor 8 (F8) inversion analysis evaluates both the intron 1 and intron 22 inversions, and is reccommended only for males with severe hemophila A, or females who have a family history of severe unknown type of hemophilia A, or females with low factor VIII levels who have no known family history of hemophilia. This assay is included in the Factor VIII (F8) severe HA Analysis-Reflex test. F8 variants other than inversions are not detected by this assay.
Additional Test Information
Amniotic Fluid, Buccal Swabs, Cultured Amniocytes, CVS, DNA, EDTA Bone Marrow, EDTA Whole Blood
3-5 mL EDTA Whole Blood or Bone Marrow (lavender top), 3-4 Buccal Swabs, 1 ug DNA at 50 ng/uL, 7-15 mL Amniotic Fluid, 5-10 mg CVS, 2-T25 flasks cultured amniocytes or CVS (2x10^6 minimum)
Fetal: Call Laboratory; Parental/Patient: 2 ml
Long-range PCR and gel electrophoresis