Also Known as
F8, Factor 8, FVIII, Hemophilia, Hemophilia A
This testing is recommended for the detection of germline variants associated with severe Hemophilia A . Testing begins with F8 inversion Analysis; if this analysis is negative, F8 Sequence Analysis is performed at an additional charge. Refer to F8 Inversion Analysis and F8 Sequence Analysis for test information.
Amniotic Fluid, Buccal Swabs, Cultured Amniocytes, CVS, DNA, EDTA Bone Marrow, EDTA Whole Blood
3-5 mL EDTA Whole Blood or Bone Marrow (lavender top), 3-4 Buccal Swabs, 1 ug DNA at 50 ng/uL, 7-15 mL Amniotic Fluid, 5-10 mg CVS, 2-T25 flasks cultured amniocytes or CVS (2x10^6 minimum)
Fetal: Call Laboratory; Parental/Patient: 2 ml
F8 Inv: 81403; F8 Seq: 81407
Capture hybridization and Next generation sequencing (MiSeq) with supplementary Sanger for quality and coverage or PCR and Bi-directional DNA sequencing.
Inversion Analysis: 5-7 days; Sequencing: 21 days