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Platelet Autoantibodies

Consistency and Reliability in the Evaluation of Idiopathic Thrombocytopenia

Review Test Description

Client Services

Email 800-245-3117 x 6250 Online Ordering Portal Hemostasis Requisition

Versiti’s lab-developed platelet autoantibody assay has been designed to ensure evaluation of ITP, without delay.


Versiti’s lab-developed platelet autoantibodies test (test code: 5544) has been designed to detect autoantibodies to platelet glycoproteins. These antibodies are eluted from the patient’s own platelets or found circulating in plasma.  

We’re committed to ensuring that your patients are receiving the best test available, without delay. That is why we have established a lab-developed ELISA assay; to guarantee that testing is available whenever you order, with an industry-leading 3-day turnaround time upon sample receipt. 

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Advantages of Versiti Platelet Autoantibodies

Our lab-developed platelet autoantibodies test helps to ensure that we’re always ready to support you and your patients. Our test method offers significant differences:

  • Detects glycoprotein-specific antibodies in eluates prepared from washed patient platelets resulting in improved specificity.6 
  • Previous tests were nonspecific in that positive results were often seen in patients with nonimmune types of thromobcytopenia.2 
  • Antibodies specific for the platelet glycoproteins GPIIb/IIIa, GPIb/IX, and GPIa/IIa are detected. 

Expertise

The Platelet & Neutrophil Immunology Laboratory (PNIL) at BloodCenter of Wisconsin, part of Versiti, is internationally known for its testing in Immune Thrombocytopenias.

Laboratory Leadership

Dr. Brian CurtisDr. Brian Curtis has provided management and scientific direction to BloodCenter of Wisconsin’s PNIL since 1999. For over 25 years, he has been involved in research investigation and clinical laboratory testing for platelet and neutrophil antibodies and antigens. Click here to read the full bio.

Industry-leading Expertise: Immune Thrombocytopenia Purpura (TP) is one of the most common causes of immune thrombocytopenia. A diagnosis of AITP is frequently reached by excluding nonimmune causes of thrombocytopenia such as sepsis, fever, acute leukemia, and drug-induced thrombocytopenia. Approximately 85% of TP patients have elevations of platelet-associated IgG (PAIgG), PAIgM or both.1 The majority of these antibodies react with platelet surface membrane glycoproteins.3-5 

Method

ELISA - Platelets and plasma are isolated from whole blood. Platelets are washed and bound autoantibodies are eluted with a low pH buffer. Eluates and patient plasma are incubated in microtiter plates coated with GPIIb/IIIa, GPIb/IX, and GPIa/IIa captured with monoclonal antibodies. Glycoprotein-bound autoantibodies (IgG/A/M) are detected with enzyme labelled antibody. Colorimetric results are measured in an ELISA reader.

References:

  1. McMillan R. N Eng J Med 304:1135-1147, 1981.
  2. Kelton JG. Thromb Haemost 74:228-, 1995.
  3. Ruyi H, Reid DM, Jones CE, Shulman NR. Blood 83:1024-1032, 1994.
  4. Brighton TA, Evans S, Castaldi PA, Chesterman CN, Chong BH. Blood 88:194-201, 1996.
  5. Berchtold P, et al. Brit J Haematol 96:477-483, 1997.
  6. Hurilmann-Forster M, Steiner B, Felten A Brit J Haematol 98:328-335, 1997.
  7. Davoren A, Bussel JB, Curtis BR, Moghaddam M, Aster RH, McFarland JG. Am J Hematol 2005;78:193-197.
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