Overview and Core Personnel
The microscopic imaging core provides reaserchers access to a wide variety of microscope systems. The Core Lab staff trains users and provides guidance with image acquisition and analysis.
Core Laboratory Operators
Marie Schulte, Ph.D.
Call: (414) 937-3857
Call: (414) 937-3842
Research Core Labs & Facilities Director:
L. William Cashdollar, Ph.D.
Call: (414) 937-3847
Instrumentation and Applications
Olympus FV1000-MPE – Laser Scanning Confocal and Multi-photon Microscope
The Olympus FV1000 MPE is equipped with 5 excitation lines: 488 nm and 515 nm which originate from a multi-line argon laser and 405 nm, 559 nm, and 635 nm which are produced by laser diodes. These excitation wavelengths cover a wide spectrum of fluorescent probes. A choice of six objectives (10x dry, 20x dry, 40x/1.3NA oil, 60x/1.2NA water, 100x/1.4NA oil, and a 40x/0.8NA water-immersion) covers a wide magnification range that couples with an increased software zoom for whole slice to intracellular visualization. The Olympus FV1000 also computer controls vertical focus in 0.01micron increments to create multi-slice, 3D images. In addition to the confocal aspects, this instrument features a Mai Tai-DeepSee infrared laser (range 690 – 1040nm) for multi-photon applications. Two Hammamatsu non-descanned detectors allow for two-color deep tissue imaging. Additionally a 25x water immersion 1.05 N.A. objective with enhanced instrument sensitivity for maximum multiphoton performance is available. This microscope enables the researchers to perform standard confocal applications on fixed and live cells as well as deep tissue and live animal imaging using the multi-photon capability. In addition, the BRI has a separate image analysis station equipped with Metamorph (Premiere 7.1).
The Nikon Ti-2 E is an inverted, high speed, motorized microscope for advanced imaging. Its major uses are for experiments using multidimensional imaging that combine multi-channel, multi-XY positions, Z-stacking, image stitching, and time-lapse imaging. Currently on the system the objectives we have are: 10x, 20x, 40x, 60x oil, and 100x oil and the filter blocks are: DAPI, GFP, Mcherry, and Cy5. It is equipped with a unique Perfect Focus System that helps users find their sample and automatically corrects focus drift in real time during a prolonged period of time-lapse imaging. Deconvolution is available in the software for noise measurement and removal. It has 2D and 3D object tracking capabilities. DIC components on the microscope enhance bright field imaging as well.
NikonTE200 with Hoffman modulation, Inverted
Used for live cell cultures and prepared mounted slides. This microscope is outfitted with PLAN Fluor objectives 2X, 4X, 10X, 20X, 40X, and 60X. Fluorescence images can be acquired using filter sets for Hoeschst, DAPI, FITC, Texas Red, and Cy5. Images can be taken with either a Diagnostic Instruments Spot RT color camera using spot advanced software or a Photometrics CoolSNAP ES camera using Metamorph 6.1 software.
Zeiss Lumar .V12, Stereoscopic microscope
The Lumar is used for specimen dissection and analysis. It uses a 1.6X or 0.8X objective allowing for high resolution of specimens and great working distance. Fluorescence filter sets allow for the excitation of GFP, CFP and Rhodamine stained samples. Images can be acquired using a 5 megapixel Zeiss AxioCam MRc 5 camera. Perform further image analysis using Zeiss AxioVision 4.5 software.