The Flow Cytometry Core contains 4 Becton Dickinson cytometer systems.
Instrumentation and Applications
BD LSRII Flow Cytometer
- Capable of 10 color and 12 parameter acquisition
- 4 laser system includes blue 488nm, red 640nm, yellow-green 561nm and UV 355nm
- BD HTS can be used on this instrument. Capable of reading 96 well and 384 well microtiter
plates
BD LSRII Special Order System
- Capable of 12 color and 14 parameter acquisition
- 4 laser system includes blue 488nm, red 640nm, green 532nm and violet 405nm
- BD HTS can be used on this instrument. Capable of reading 96 well and 384 well microtiter plates
BD FACSAria Cell Sorter
- Capable of 10 color and 12 parameter acquisition
- 4 laser system includes blue 488nm, red 633nm, green 532nm and violet 407nm
BD FACS Melody Cell Sorter
- Capable of 9 color and 10 parameter acquisition
- 4 laser system includes blue 488nm, red 640nm and violet 405nm
- Users are trained on this instrument by our Flow Cytometry specialist so they can perform their own cell sorts
Accuri C6
- Capable of 4 color and 6 parameter acquisition
- 2 laser system includes blue 488nm and red 633nm
The core provides data analysis with the following software:
- FlowJo
- FCS Express
- FACSDiva
- CFlow Plus
Analysis workstations and training are available for Flow Core users.
Miltenyi Biotec Magnetic Cell Separation Workstation
This workstation consists of a stand and four separation magnets that can be reserved for use. The usually vast initial cell population can be reduced and enriched for the target cell, with little or no added abuse to the cells. This cuts sorting time and usually increases viability.
Scheduling
The Flow Core provides these services to all its investigators and outside users, as capacity permits. Instruments are reserved on a first-come, first-served basis with priority given to BRI Investigators. Any new users to the Core are required to attend a free training session. All BD FACSAria Sorts are operator assisted and therefore must be scheduled at least 24 hours in advance. As a courtesy, we require 24 hours notice to cancel a sort, so that time can be made available to other users.
Sample Preparation
Please discuss your experimental protocol with us. Sample preparation is always critical to success, and some reagents are not recommended due to optical considerations. We need to determine if there are any special cytometer cleaning requirements. The handling of any infectious or otherwise dangerous materials should be discussed prior to booking time on any system.